Juventix Regenerative Medical – The Difference is the Science
Platelet Rich Fibrin is an advanced form of platelet concentrates utilized in various medical, aesthetic and dental procedures for regenerative properties. Platelet Rich Fibrin is called the “second generation of platelet products”. It is a unique structure whereas platelet rich plasma is inflammatory, platelet rich fibrin is anti-inflammatory.
Platelet Rich Fibrin has garnered significant attention in regenerative medicine due to the anti-inflammatory abilities and the enhanced tissue regeneration over a longer duration due to the matrix formation. It is prepared from platelet rich plasma. When exposed to certain conditions and stimuli, platelet rich plasma transforms into a dense fibrin network, producing platelet rich fibrin. This is a thicker denser material which makes it ideal for bio filler in aesthetic usage or longer acting treatment in musculoskeletal applications.
The initial inventor applied platelet rich fibrin in dental applications where it is still being used today in dental surgical procedures.
Three Methods to Create Platelet Rich Fibrin
Platelet Rich Fibrin can be made from platelet rich plasma by three methods.
A long centrifuge process where the plasma separates from the denser red blood cells. This upper layer that results is a solid formation of platelet rich fibrin that is extracted from the centrifugation tube and then dissected into pieces which is commonly used in dentistry to pack an extraction site. The PRF promotes healing and is anti-infective.
The second method to make platelet rich fibrin is to add an activator to release the growth factors, cytokines and bioactive protein from their granules contained on the platelets. The activator is commonly calcium chloride or thrombin. This activation process results in a dense fibrin matrix that ultimately becomes extremely hard.
The third method to produce platelet rich fibrin is the heating method. Once removed from the centrifuge, the platelet rich plasma concentrate is subjected to a heat source. This is a novel modification of the overall process aimed at enhancing the properties of fibrin and altering the matrix architecture.
Understanding the temperature thresholds and exposure time is critical during the preparation of heat generated platelet rich fibrin as excessive heat will cause denaturation of the proteins compromising the functional properties.
Methods for Generating Heat Generated Platelet Rich Fibrin
Platelet Rich Plasma is first obtained from a blood sample to isolate the platelets. This is accomplished through a centrifugation procedure. The blood sample will separate into three distinct layers. The lower and most dense are the red blood cells, the middle layer is the buffy coat layer where the white blood cells and platelets reside, and the uppermost layer is the plasma.
With the use of a thixotropic gel separator tube, the red cells and most of the white cells are trapped below the gel. The uppermost layer contains the platelet rich plasma at the base and the platelet poor plasma above.
This simplifies the process to obtain the platelet rich plasma just above the gel for transition into platelet rich fibrin.
Heat Induced Fibrin Formation – The PRP is subjected to a controlled heating process to initiate fibrin formation. Typically, the heat is applied at a temperature range of not greater than 60 degrees centigrade. The general time is 5-15 minutes.
Research has suggested that temperatures about 42–47°C facilitate optimal fibrin formation without excessive denaturation of the proteins. At temperatures above 60°C, proteins such as fibrinogen begin to denature leading to a reduction in the fibrin network quality and the release of growth factors.
The denature point for fibrinogen is approximately 60°C, above which the protein undergoes structural changes that render it non-functional. Therefore, maintaining temperatures below 60°C is critical for preserving the biological activity of the growth factors and fibrinogen.
Heating times play a significant role in the final fibrin matrix network. The optimal heating time is 10–15 minutes. This time dictates the density of the final product and can be manipulated for therapeutic usage. Prolonged time denatures fibrinogen, reducing the quality and activity of the fibrin cascade mechanism.
Effects of Heat on Protein Denaturation
Fibrinogen is a glycoprotein involved in blood clotting and is a central actor in the formation of the fibrin matrix. High heat causes fibrinogen to undergo a conformational change in its structure, reducing or completely prohibiting its ability to polymerize into fibrin. Temperatures above 60°C break down fibrinogen and prevent fibrin network formation.
Platelets in PRP release a variety of growth factors that aid in tissue regeneration. Elevated temperature inhibits the release and activity of these proteins. The most common growth factors contained on the platelets are platelet derived growth factor and transforming growth factor-beta. Both are sensitive to heat, and prolonged temperatures above 55–60°C cause denaturation, reducing their regenerative capacity.
Other cytokines and growth factors have been found to be sensitive to excessive heat, leading to diminished activity.
Activation of Platelet Rich Plasma
Activation of platelet rich plasma is the process by which the platelets release their growth factors, cytokines and bioactive proteins contained on the granules of the platelets. PRP relies on tissue damage or exogenous additives to accomplish this process. Some researchers believe the centrifugation process releases minute amounts of calcium chloride which reside on the lambda granules on the platelets. Others count on additives such as calcium chloride, thrombin or collagen to precipitate the activation.
In cases where calcium chloride has been added to the PRP to enhance the release of growth factors and form the fibrin network, this form of activation should be done judiciously as premature excessive activation may cause premature clotting. Since the FDA has banned additives in their minimal manipulation doctrine of 2021, activation is now accomplished by polychromatic light.
Debunking Fallacies About PPP and PRP Heating Protocols
A significant misconception in the preparation of platelet rich fibrin revolves around certain protocols that first centrifuge blood, then separate the platelet rich plasma from platelet poor plasma. This is followed by the absence of activation, but an excessive heating process and subsequently after heating, mixing the heated product with the platelet rich plasma and labeling this concoction as platelet rich fibrin.
This is not true platelet rich fibrin as it involves the denaturation of proteins and a total lack of a true fibrin matrix.
This process of excessive heating of PPP causes the denaturation of the proteins including albumin. The denatured proteins are rendered ineffective in their original function. The formation of the matrix structure is negated and without the fibrin structure, one cannot assume it is platelet rich fibrin but only heated denatured proteins, albumin and the mixed platelet rich plasma from the initial division of the centrifuged blood.
The lack of activation and the absence of the fibrin matrix means that the growth factors are not released in a sustained or controlled manner, which reduces the therapeutic potential of this product.
Studies Supporting True Platelet Rich Fibrin Formation
In Vivo Assessment of Plasma Gel: Regenerative Potential and Limitations as a Filler
J Cosmet Dermatol 2025 Feb 24(2): e16765 PMID 39918180
Background: This gel is created by heating platelet poor plasma to induce denaturation forming a gel like substance when combined with platelet rich plasma.
Aims: This study aims to investigate the in vivo behavior of plasma gel, focusing on its absorption rate and its ability to stimulate tissue regeneration.
Results: The study found that plasma gel is absorbed rapidly with approximately 50% of the volume disappearing within the first week, 90% absorbed by day 28 and complete absorption by 8 weeks.
Conclusion: The main findings suggest plasma gel is quickly absorbed and may not be suitable for long-term volumizing effects, limiting its effectiveness as a long-term filler.
Note: The quick degradation of plasma gel is largely due to the fact that PPP contains a significant proportion of water and denatured proteins after excessive heating.
Reference Studies
- Platelet Rich Fibrin in Dentistry
J Appl Biomater Funct Mater 2024 Jan-Dec;22;22808000241299588 PMID 39588592 - Denaturation and Its Effect on Fibrinogen Activity
J Tissue Engineering 2016, 8(2), 101-109 - Impact of Temperature on Fibrinogen and Platelet Rich Plasma Quality
Platelets 2017, 28(7), 641-648 - Classification of Platelet Concentrates: From Pure PRP to Leukocyte and Platelet Rich Fibrin
Trends in Biotechnology 2009;27(3):158-167 PMID 19187989
Juventix Regenerative Medical: Science-Based PRP and PRF
Juventix Regenerative Medical is an industry leader in the regenerative medical field for a reason. Juventix Regenerative Medical follows the science. Our Platelet Rich Plasma Kits are designed with a thixotrophic gel separator that is molecularly weighted to allow approximately 9% leukocytes into the platelet rich plasma. Leukocytes are a significant component of the regeneration of tissues.
Juventix Regenerative Medical activates the platelets to release the growth factors, cytokines and bioactive proteins to be released from the granules contained on the platelets. This activation is uniform and is accomplished by LED light in our patented LED Activator. No chemicals are added such as calcium chloride or thrombin and the entire sample is activated by the poly chromatic light.
Juventix Regenerative Medical does not separate the activated platelet rich plasma from the platelet poor plasma in many protocols. The sample is placed in our patented Bio-Incubator for transformation from an inflammatory platelet rich plasma to an anti-inflammatory platelet rich fibrin. This is accomplished through a heating process in a patented ceramic block heating device that shields the sample for uniform heating and subsequent cooling. Other manufactures use an aluminum block heating process that does not uniformly treat the sample and can damage and lyse the cells due to the temperatures used.
Juventix Regenerative Medical employs lower temperatures to transition the platelet rich plasma into platelet rich fibrin while not denaturing the proteins, harming the fibrinogen and impairing the actions of the growth factors.
The use of these devices and our protocols follows the science of platelet rich plasma and platelet rich fibrin. Juventix is clearly different from the competition with results that speak for themselves.
Your Partner in Advanced Regenerative Science
Juventix Regenerative Medical has a vast array of products, devices and services tailored for the regenerative professional. All our products and devices follow the science which separates us from the competition on many levels. All devices and products from Juventix are FDA cleared, approved or registered.
The regenerative marketplace is exploding with technological advancements being added daily for both men and women in all applications and fields of medicine and aesthetics. With our premier line of products, services and devices and guidance through our experienced staff, Juventix will aid and guide any regenerative professional in providing enhanced outcomes and increased patient satisfaction.
RESTORE, REVIVE, REGENERATE – JUVENTIX REGENERATIVE MEDICAL
Regenerative Regards,
Dr. Robert McGrath
